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. 2019 Apr 12;17(4):223. doi: 10.3390/md17040223

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE analysis of differentially salt (NaCl)-precipitated collagens from hoki cartilage. Lanes 2–5, from pepsin-soluble fraction. Lanes 6–9, from alkaline-soluble fraction. Lanes 1 and 10, Broad range marker; lanes 2 and 6, proteins precipitated by 0.8 M NaCl; lane 3, proteins precipitated by 1.0 M NaCl; lane 7, proteins precipitated by 1.2 M NaCl; lanes 4 and 8, proteins precipitated by 2 M; lanes 5 and 9, same proteins as in lanes 4 and 8 after incubation with a reducing agent.