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. 2019 Apr 24;11(4):378. doi: 10.3390/v11040378

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FUT8-specific siRNA and recombinant expression vector were designed and verified. (A) qRT-PCR and Western blotting analysis were used to detect the relative hepatitis C virus (HCV) RNA expression and non-structural protein 3 (NS3) protein level 72 h post HCV infection in Huh7.5.1 cells. Mock: only Huh7.5.1 cells. The specificity of the FUT8 siRNA was confirmed by RT-PCR (B) and Western blot (C). Statistical analyses of (C) are listed in (D). (E) Overexpression of FUT8 was confirmed by Western blot after transfection with FUT8 plasmid (pcDNA3.1-FUT8, named pc3.1-FU8). Statistical analyses of (E) are also listed in (F).