Protective effects of GBE against alcohol-induced hepatic apoptosis and oxidative stress. Representative immunohistochemical staining of liver for (A) terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (green) and (B) cleaved caspase-3 (red) with corresponding quantitative data. (C) Western blot of hepatic cleaved poly (ADP-ribose) polymerase (PARP), lamin B, cleaved caspase-3, B-cell lymphoma 2 (Bcl-2), and p53 expression with quantitative data. Representative immunohistochemical staining of liver for (D) 8-hydroxy-2’-deoxyguanosine (8-OHdG) (green) and (E) malondialdehyde (red) with corresponding quantitative data. CON, control; SIL, silymarin; GBE, water extract of G. bimaculatus. Data are shown as means ± SEM (n = 4 for A, B, D and E and n = 8 for C). * p < 0.05 vs. EtOH control group.