Table 3.

Effects of rosmarinic acid and sinapic acid on the glutathione-related enzymes activities in the lenses of ovariectomized rats.

Parameter/Group	SHAM	OVX	OVX + ESTR	OVX + RA10	OVX + RA50	OVX + SA5	OVX + SA25
GPx (nmol/min/mg of protein)	2.31 ± 0.08	2.15 ± 0.08	2.04 ± 0.07	2.26 ± 0.11	2.21 ± 0.06	2.24 ± 0.08	2.28 ± 0.05
GR (nmol/min/mg of protein)	0.367 ± 0.047	0.220 ± 0.036 **	0.291 ± 0.034	0.357 ± 0.027 ^^	0.352 ± 0.026 ^^	0.323 ± 0.026 ^	0.346 ± 0.021 ^
G6PD (nmol/min/mg of protein)	1.99 ± 0.10	1.30 ± 0.14*	1.39 ± 0.17 *	1.52 ± 0.19	1.83 ± 0.16	1.47 ± 0.13	2.00 ± 0.27 ^
GGT (nmol/min/mg of protein)	0.039 ± 0.005	0.024 ± 0.004*	0.028 ± 0.003	0.024 ± 0.004 *	0.020 ± 0.002 **	0.025 ± 0.005 *	0.031 ± 0.002

Rosmarinic acid at doses of 10 mg/kg (OVX + RA10) and 50 mg/kg (OVX + RA50), sinapic acid at doses of 5 mg/kg (OVX + SA5) and 25 mg/kg (OVX + SA25) or estradiol at a dose 0.2 mg/kg (OVX + ESTR) were administered orally to ovariectomized rats, once daily for 28 days. SHAM: sham-operated control rats; OVX: ovariectomized control rats; GPx: glutathione peroxidase; GR: glutathione reductase, G6PD: glucose-6-phosphate dehydrogenase. Results are presented as the mean ± SEM. One-way ANOVA followed by Duncan’s test was used for evaluation of the significance of the results. * p ≤ 0.05, ** p < 0.01: significant differences with regard to the SHAM control rats. ^ p ≤ 0.05, ^^ p < 0.01: significant differences with regard to the OVX control rats. No statistically significant differences in results for GPx were demonstrated by ANOVA.