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. 2019 Mar 28;11(4):439. doi: 10.3390/cancers11040439

Figure 2.

Figure 2

GSH inhibits caspase induction after treatment with melphalan. Induction of caspases was monitored after treatment with melphalan with a luciferase assay. (A) Activation of caspases after treatment of XG7 cells. XG7 cells were treated with 15 µM of melphalan with or without cotreatment with GSH (5 mM) and induction of caspases was evaluated at day 1, 2 and 3 post-treatment with a luciferase assay. Graphs shows results of bioluminescence intensity for caspase assay. Bioluminescence intensity resulting from the addition of Cell titer glo assay reagent and measuring ATP concentration and therefore reflecting the count of viable cells was used for normalization. Results for activation of caspase 3/7 are shown in the left panel and for caspase 9 in the right panel. (B) caspase activation after treatment of XG2 cell line. Same experiment after treatment of XG2 cells with 5 µM melphalan. ** p < 0.05 using a Wilcoxon test for pairs.