Figure 4.

Ascophyllan enhances the cytotoxic activity of NK cells. C57BL/6 mice were administered either ascophyllan (Asco, 50 mg/kg) or fucoidan (Fuco, 50 mg/kg) by i.p. injection, twice, separated by 24 h. Ascophyllan- or fucoidan-stimulated spleen NK cells (effector cells) were isolated from mice 24 h after the last injection and co-cultured with Yac-1 cells (target cells) at the indicated ratios. Cytotoxicity was measured after 6 h of co-culture by the lactate dehydrogenase (LDH) assay. Mean ± standard error of the mean. (n = 6) ** p < 0.01.