Table 2.
Analyzed embryo characteristics and clinical outcomes
| Blastomere movement type | ||||
|---|---|---|---|---|
| Total | Bouncing | Wobbling | Twist-and-crumble | |
| No. of cleaved embryos analyzed | 966 | 543 (56.2) | 194 (20.1) | 229 (23.7) |
| Four-cell stage embryos | 964 (99.8) | 543 (100) | 194 (100) | 228 (99.6) |
| Eight-cell stage embryos | 900 (93.2) | 516 (95.0)a | 181 (93.3)a | 202 (88.2)b |
| Morula | 842 (87.2) | 493 (90.8)a | 167 (86.1)a,b | 180 (78.6)b |
| Blastocysts | 731 (75.7) | 435 (80.1)a | 144 (74.2)a,b | 152 (66.4)b |
| Blastocysts expanded | 681 (70.5) | 406 (74.8)a | 136 (70.1)a,b | 139 (60.7)b |
| Blastocysts cryopreserved | 625 (64.7) | 378 (69.6)a | 124 (63.9)a | 123 (53.7)b |
| Morphological grade of blastocysts | ||||
| ICM | ||||
| Grade A | 291 (46.6) | 184 (48.7) | 59 (47.6) | 48 (39.0) |
| Grade B | 214 (34.2) | 129 (34.1) | 37 (29.8) | 48 (39.0) |
| Grade C | 120 (19.2) | 65 (17.2) | 28 (22.6) | 27 (22.0) |
| TE | ||||
| Grade A | 267 (42.7) | 177 (46.8)a | 56 (45.2)a | 34 (27.6)b |
| Grade B | 178 (28.5) | 97 (25.7)a | 35 (28.2)a,b | 46 (37.4)b |
| Grade C | 180 (28.8) | 104 (27.5) | 33 (26.6) | 43 (35.0) |
| No. of blastocysts thawed | 499 | 298 | 102 | 99 |
| No. of survived blastocysts | 496 (99.3) | 297 (99.7) | 101 (99.0) | 98 (99.0) |
| No. of blastocysts transferred | 496 | 297 | 101 | 98 |
| Morphological grade of blastocysts transferred | ||||
| ICM | ||||
| Grade A | 250 (50.3) | 157 (52.7) | 52 (51.5) | 41 (41.8) |
| Grade B | 158 (31.9) | 90 (30.4) | 33 (32.7) | 35 (35.7) |
| Grade C | 88 (17.8) | 50 (16.9) | 16 (15.8) | 22 (22.5) |
| TE | ||||
| Grade A | 235 (47.3) | 155 (52.0)a | 50 (49.5)a | 30 (30.6)b |
| Grade B | 128 (25.9) | 65 (22.0)a | 27 (26.7)a,b | 36 (36.7)b |
| Grade C | 133 (26.9) | 77 (26.0) | 24 (23.8) | 32 (32.7) |
| Clinical pregnancies | 234 (47.2) | 136 (45.8) | 47 (46.5) | 51 (52.0) |
| Ongoing pregnancies | 201 (40.5) | 115 (38.7) | 41 (40.6) | 45 (45.9) |
| Follow-up data on live birth (ET date: April–December 2017) | 246 | 149 | 53 | 44 |
| Live birth | 88 (35.8) | 52 (34.9) | 18 (34.0) | 18 (40.9) |
| dBMov | 3.84 ± 0.07 [0.16–25.54] | 2.82 ± 0.05a [0.16–8.49] | 5.20 ± 0.13b [0.734–14.57] | 5.36 ± 0.17b [0.63–25.54] |
| dBMov/(t3-t2) | 0.366 ± 0.007 [0.014–1.000] | 0.253 ± 0.004a [0.014–0.727] | 0.497 ± 0.015b [0.071–1.000] | 0.523 ± 0.016b [0.061–1.000] |
Categorical data are presented as n (%), continuous data are presented as mean ± standard error of the mean [range]. Different superscript letters (a, b) indicate a significant difference at P < 0.05. Abbreviations: dBMov, blastomere movement duration; dBMov/(t3-t2), ratio of the duration of blastomere movement at the 2-cell stage; ICM, inner cell mas; ET, embryo transfer; TE, trophectoderm