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. 2019 May 15;17:44. doi: 10.1186/s12964-019-0355-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — MIIP negatively regulates AKT-mTOR signaling. (a, b) Western blot analysis of key components of PI3K-AKT-mTOR signaling pathway [PI3K (p110), AKT, p-AKT (Ser473), p-AKT (Thr308), p-mTOR (Ser2481) and p-mTOR (Ser2448)] in stable control or MIIP-overexpressing LNCaP and C4–2 cells, and in LNCaP and 22Rv1 cells transfected with siNC or two individual MIIP-targeting siRNAs . (c) Western blot analysis of p-AKT (Ser473), p-AKT (Thr308), p-mTOR (Ser2481) and p-mTOR (Ser2448) in stable control and MIIP-overexpressing LNCaP and PC3 cells treated with BKM120 (0, 100 nM, 500 nM, or 1 μM). Each band’s intensity was quantified by Image J software, with the relative values (GAPDH or tubulin as internal control) of the left-most band being designated as 1 and the values of the others relative to it shown below