Figure 2.

Qingshen Buyang Formula decreased the expression of collagen I and FN. ((a) and (b)) Immunofluorescence and immunohistochemistry staining demonstrated that the protein expression of collagen I and FN, which are the most important components of ECM, was inhibited by Qingshen Buyang Formula. Scale bar, 50μm. ((c) and (d)) Graphic presentation of mRNA expression of collagen I and FN demonstrated significant difference among the three groups. ∗ P< 0.05, compared with the Sham group. # P< 0.05, compared with the 5/6Nx group. The error bars represent the standard deviation of measurements for 8 particles in three separate sample runs (n = 24).