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. 2019 May 2;2019:5370847. doi: 10.1155/2019/5370847

Figure 4.

Figure 4

Qingshen Buyang Formula inhibited activated Wnt/β-catenin signaling pathway. (a) Representative western blot analyses illustrated that Qingshen Buyang Formula suppressed the protein expression of Wnt4, TCF4, and p-GSK3β involved in Wnt/β-catenin signaling pathway, while the expression of GSK3β had no change. ((b) and (c)) Graphic presentation of Wnt4, TCF4, and p-GSK3β/GSK3β in the three groups as indicated. ((d)–(f)) Real-time PCR revealed that Qingshen Buyang Formula reversed the increased mRNA expression of Wnt4, TCF4, and β-catenin. ∗ P< 0.05, compared with the Sham group. # P< 0.05, compared with the 5/6Nx group. The error bars represent the standard deviation of measurements for 8 particles in three separate sample runs (n = 24).