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. 2019 May 9;10:1012. doi: 10.3389/fmicb.2019.01012

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Copyright © 2019 Rane, Hayek, Frye, Abeyta, Bernardo, Parra and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth and cytosolic pH in Pma1p truncation mutants. Growth and cytosolic pH were measured concurrently in Pma1p truncation mutants by growing cells in YNB with 2% glucose in a plate reader with OD₆₀₀ readings and cytosolic pH readings taken at 15 min intervals. Cytosolic pH is plotted on the left y-axis and is represented by closed circles. OD₆₀₀ is plotted on the right y-axis and is represented by + symbols. Increased Pma1 truncation is correlated with decreased growth and increased cytosolic acidification. Graph represents the average of four individual experiments.