Table 1.

Pma1p truncation masks the effects of bafilomycin A1 on growth.

	-bafilomycin	+bafilomycin
BWP17+CIp30	0.2173 ± 0.0010	0.1745 ± 0.0009∗
Δ878	0.2214 ± 0.0010	0.2117 ± 0.0013∗
Δ866	0.2170 ± 0.0009	0.1979 ± 0.0007∗
Δ864	0.1792 ± 0.0008	0.1675 ± 0.0009∗
Δ862	0.1947 ± 0.0009	0.1914 ± 0.0009∗
Δ858	0.1306 ± 0.0011	0.1311 ± 0.0008

Growth rates with and without the V-ATPase specific inhibitor bafilomycin were measured in Pma1p truncation mutants by growing cells in CSM with 2% glucose in a plate reader with OD₆₀₀ readings taken at 15 min intervals. Growth curves were analyzed using Graphpad Prism: first, curves were fitted using a Weibull growth model (Weibull, 1951). Then, calculated growth rates were analyzed two-way ANOVA, as appropriate, followed by multiple-comparison post-tests. Data represents the average values of three independent experiments. Addition of bafilomycin did not reduce growth in the Δ858 strain (truncated by 38 aa, respectively). ^∗Indicate statistical significance compared to the growth rate of the same strain without bafilomycin.