Table 2. Detection of phosphatidylserine expression by Annexin V method on L.(L) amazonensis promatigotes exposed to different concentrations of nitro-heterocyclic derivatives and amphotericin B.
| PI+ (%) | AV+ (%) | PI+ AV+ (%) | |
|---|---|---|---|
| Negative Control | 2.13 | 2.94 | 0.86 |
| DMSO PA Control | 3.75 | 1.15 | 0.27 |
| [100 µM] | |||
| Amphotericin B | 5.43 | 16.8 | 1.68 |
| BSF-C4H9 | 4.56 | 11.7 | 4.11 |
| BSF-CH3 | 7.63 | 7.86 | 3.56 |
| BSF-Cl | 11.2 | 14.5 | 5.42 |
| BSF-H | 2.61 | 25.1 | 4.1 |
| BSF-NO2 | 7.67 | 1.25 | 0.29 |
| [25 µM] | |||
| Amphotericin B | 6.72 | 35.1 | 3.03 |
| BSF-C4H9 | 4.06 | 5.87 | 3.52 |
| BSF-CH3 | 3.64 | 3.87 | 0.93 |
| BSF-Cl | 4.68 | 19 | 6.2 |
| BSF-H | 2.49 | 14.3 | 4.06 |
| BSF-NO2 | 1.22 | 9.28 | 2.21 |
| [6.25 µM] | |||
| Amphotericin B | 2.42 | 48.3 | 3.88 |
| BSF-C4H9 | 1.04 | 7.19 | 2.11 |
| BSF-CH3 | 0.94 | 6.21 | 1.58 |
| BSF-Cl | 1.33 | 7.53 | 1.97 |
| BSF-H | 2.23 | 7.53 | 1.96 |
| BSF-NO2 | 1.72 | 2.7 | 0.67 |
Data expressed by the percentage of the average fluorescence intensity of Annexin V-FITC and Propidium Iodide-PE on L.(L) amazonensis promatigotes. Incubation for 48 hours, at 32 °C and 5% of CO2. AV+ (Q3): Annexin V positive; PI+ (Q1): Propidium iodide positive PI+ AV + (Q2): Annexin V and Propidium iodide positive; Negative control: promastigotes untreated.