Skip to main content
. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer Cell. 2019 Apr 4;35(4):633–648.e7. doi: 10.1016/j.ccell.2019.03.003

Figure 2. Function of UHRF1 domains in maintaining DNA hypermethylation-associated gene silencing in CRC cells.

Figure 2.

(A) Heatmap showing relative gene expression (log2 fold changes relative to Mock) of the top 500 most differentially expressed genes in endogenous UHRF1 depleted HCT116 (left) and RKO cells (right) complemented by the indicated mutants.

(B) Relationship between gene upregulation and promoter DNA demethylation in endogenous UHRF1 depleted HCT116 (top) and RKO cells (bottom) complemented by the indicated mutants. The x-axis denotes the relative gene expression changes (log2 fold changes relative to Mock), and the orange vertical lines in each panel represent the log2 fold changes equal to −1 and 1, respectively. The y-axis denotes promoter DNA methylation changes defined by the difference of the mean β (Δβ) values of the promoter probes for each individual gene with respect to Mock.

(C) Venn diagrams showing genes with promoter demethylation (Δβ value < −0.2 compared to Mock) and upregulation (log2 fold change > 1 compared to Mock) in HCT116 (left) and RKO cells (right) complemented by EV and the representative UHRF1 domain mutants along with endogenous UHRF1 depletion.

See also Figure S2.