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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer Cell. 2019 Apr 4;35(4):633–648.e7. doi: 10.1016/j.ccell.2019.03.003

Figure 4. UHRF1 contributes to CRC metastasis in part through the maintenance of TSGs silencing.

Figure 4.

(A) Transwell assay analyses of the migration and invasion capabilities of SW620 cells simultaneously depleting UHRF1 and the indicated genes. Values shown are the mean of three replicates.

(B–G) A mouse model of lung colonization was established by tail vein injection of SW620 cells simultaneously depleting UHRF1 and the indicated proteins into the immune-incompetent mice (n = 10), followed by noninvasive bioluminescence imaging for 9 weeks. Representative bioluminescent imaging (B), bioluminescence signals (C), overall survival (D), incidence of lung colonization (E), the number of lung colonization foci (F), and representative hematoxylin and eosin (H&E) staining of lung tissues (G) from the different groups is shown. In (G), the boxed regions in the top images (scale bars, 1 mm) are magnified in the lower images (scale bars, 100 μm).

(H–M) A mouse model of liver metastases was established by intrasplenic injection of SW620 cells simultaneously depleting UHRF1 and the indicated proteins into the immune-incompetent mice (n = 10). Representative bioluminescent imaging recorded at 9 weeks after injection (H), bioluminescence signals recorded at the indicated time points (I), overall survival (J), incidence of liver metastases (K), number of tumor foci on the liver surface (L), and representative H&E staining of liver tissues (M) from the different groups is shown. Scale bars, 200 μm.

Data are represented as mean ± SEM. *p < 0.05. See also Figure S4, Tables S1 and S2.