Figure 5. Higher UHRF1 levels are associated with increased aggressiveness of human CRC.
(A-D) Real-time RT-PCR analyses of UHRF1 mRNA expression levels in normal colon epithelial tissues (n = 20), 120 paired, adjacent non-tumor and CRC tissues (A); in CRC samples from patients with recurrence (n = 69) or without recurrence (n = 51) (B); in CRC samples from patients with metastases (n = 57) or without metastases (n = 57) (C); in primary colon cancer tissues and paired metastatic colon cancer tissues (n = 30) (D). Data are represented as mean ± SEM. *p < 0.05.
(E) Representative IHC staining of UHRF1 in adjacent non-tumor tissues and CRC tissues. The boxed regions in the top images are magnified in the lower images. Scale bars represent 200 μm (top) and 50 μm (bottom).
(F) Comparison of UHRF1 protein levels in CRC tissues and adjacent non-tumor tissues in two independent patient cohorts based on the IHC scores. Data are represented as mean ± SEM. *p < 0.05.
(G) Representative IHC staining of UHRF1 in adjacent non-tumor tissues, primary colon cancer tissues and the tissues with lymph node metastases of the same patient. The boxed regions in the top images are magnified in the lower images. Scale bars represent 500 μm (top) and 50 μm (bottom).
(H) Kaplan-Meier analyses of the correlation between UHRF1 expression and the recurrence or overall survival of CRC patients in two independent cohorts.