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. 2019 May 9;9:369. doi: 10.3389/fonc.2019.00369

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Copyright © 2019 Rong, Li, He, Liu, Ren, Gao, Mu, Guan, Duan, Zhang, Zhang, Li, Deng and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FTO mRNA level was low in ICC cells. (A) The genetic alterations of erasers, readers, and writers for the m⁶A modification in ICC were analyzed by cBioPortal. (B) The expression levels of erasers, readers and writers for m⁶A modification in ICC cell lines (n = 4) and the intrahepatic biliary epithelial cell line HIBEPIC were analyzed by q-PCR. (C) The mRNA levels of FTO in control, overexpression, and knockdown TFK1 cells were assessed by q-PCR. (D,E) The protein levels of FTO in control, overexpression and knockdown TFK1 and HCCC-9810 cells.