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. 2019 May 9;10:491. doi: 10.3389/fphar.2019.00491

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Copyright © 2019 Zhang, Wang, Li, Dai, Li, Wang, Wang, Ma, Gu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study design for the investigation of untargeted metabolomics using hydrophilic interaction liquid chromatography (HILIC), reverse phase liquid chromatography coupled with mass spectrometry (RP/UHPLC/MS), and a yeast-induced febrile rat model to explore the antipyretic mechanism of Gardeniae Fructus (GF), prepared GF (GFP), and carbonized GF (GFC).