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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Circ Res. 2019 Mar 15;124(6):891–903. doi: 10.1161/CIRCRESAHA.118.314560

Figure 2: Endothelial talin is required for maintenance of intestinal vascular integrity and barrier function.

Figure 2:

A. TdTomato and FITC-lectin were visualized in the villi of mice 16 days after tamoxifen injection. Mice were injected intravenously with FITC-Lectin 30 minutes prior to sacrifice. (n=3;scale=50 μm). Total FITC-Lectin fluorescence and intravascular lectin levels were quantitated indicating increased extravascular leak in Tln1 EC-KO-tdTom mice relative to Tln1 CTRL-tdTom (n=3 mice/group; *p=0.039 two-tailed unpaired t-test) B. Confocal microscopic analysis of cryosections of intestine showing tdTomoto fluorescence and collagen IV immunofluorescence. Inset shows a zoomed region demonstrating endothelial cell rounding (white arrows) and detachment from neighboring cells in the intestinal villi of Tln1 EC-KO-tdTom mice. (n=3; scale=50 μm; zoom scale=10 μm). C. TdTomato fluorescence showing disorganized capillaries and cyst-like structures (white arrows) in Tln1 EC-KO-tdTom intestinal wall and villi 12 days after tamoxifen injections. (n=3; scale=100 μm).