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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Circ Res. 2019 Mar 15;124(6):891–903. doi: 10.1161/CIRCRESAHA.118.314560

Figure 7: Talin-dependent integrin activation is indispensable for endothelial adherens junction organization.

Figure 7:

A. Antibody internalization assay in which mouse VE-Cadherin (red) antibody was incubated with either shTln1 or shScramble HUVECs for 30min prior to fixation after which excess surface-bound antibody was removed by acid washing as described in Methods. After fixation/permeabilization, total levels of VE-Cadherin (green) were visualized using a rabbit VE-Cadherin antibody. (n=3; scale= 25 μm; **p=0.0077 two-tailed unpaired t-test). B. shTln1 HUVECs were transfected with either Empty-GFP, GFP-Talin1, GFP-THD or GFP-THD-L325R after which junctional width was assessed as described above and in Methods. GFP-Talin1 (GFP+) and GFP-Talin1 (GFP-) cells junctional widths were quantitated as an internal control for transfected groups. (n=3; scale=25 μm; *** p=0.0007, ** p=0.0099, ns=not significant, one-way ANOVA with a Tukey multiple comparisons test.