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. Author manuscript; available in PMC: 2019 May 16.
Published in final edited form as: Cell Rep. 2019 Mar 26;26(13):3537–3550.e4. doi: 10.1016/j.celrep.2019.03.005

Figure 6. NFATc3 Does Not Translocate from Distal Dendrites to the Nucleus after a Brief Uncaging Stimulus.

Figure 6.

(A) Representative neuron transfected with mEos3.2-NFATc3 with preconversion green and red species and red species after conversion within distal dendrites indicated with dotted lines on the left.

(B) Images of soma showing mEos3.2-NFATc3 distribution before (pre) and after photo-conversion in control conditions (−MNI-glu). Top: green species; bottom: red species. Images are from the same neuron in (A).

(C) mEos3.2-NFATc3 nuc/cyto normalized to pre-uncaging values following branch photoconversion in 6–7 ROIs without MNI-glu in the bath. Purple arrows indicate photoconversion within a dendritic ROI (n = 6).

(D–F) As in (A)–(C) but from experiments with MNI-glu in the bath (+MNI-glu; n = 7; one-way ANOVA repeated-measures with Dunnett, ***p < 0.001).

(G–I) As in (A)–(C) but following photoconversion within a single ROI drawn on the soma (−MNI-glu; n = 5; one-way ANOVA repeated-measures with Dunnett, ***p < 0.001, **p < 0.01, *p < 0.05).

(J–L) As in (G)–(I) but following photoconversion within a single ROI drawn on the soma with MNI-glu in the path (+MNI-glu; n = 5; one-way ANOVA repeated-measures with Dunnett, ***p < 0.001, **p < 0.01). Scale bars = 20 μm.

See also Figure S6.