Figure 2.

Activating the T-cell type 1 angiotensin receptor suppresses accumulation of CD4⁺ T cells in obstructed kidney. A and B: Representative flow cytometry gating strategy for single cell suspensions from unilateral ureteral obstruction kidney. Live cells were gated for CD45⁺population, then single cells, then CD11b⁺ Ly6G^- cells, as indicated by boxes and arrows. Among these CD11b⁺ Ly6G^- cells, Ly6C^hi populations were isolated, as indicated by boxes. C–E: Cell number of CD4⁺CD8⁻ or CD4⁻CD8⁺ T lymphocytes in obstructed kidneys of Lck-Cre Agtra^flox/flox [total knockout (TKO)] and wild-type (WT) mice. F: mRNA expression of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, IL-1β, and IL-17 in WT and TKO obstructed kidneys. ^∗P < 0.05 versus obstructed WT. au, arbitrary units; FSC-A, forward scatter area; FSC-H, forward scatter height; SSC-A, side scatter area.