Figure 3.

Type 1 angiotensin (AT₁) receptors on T lymphocytes inhibit the infiltration of proinflammatory monocytes into the fibrosing kidney. A and B: Representative flow cytometry gating strategy. Live cells were gated for CD45⁺population, then single cells, then CD3⁺T cells, as indicated by boxes and arrows. CD3⁺ T cells were separated into CD4 and CD8 single positive populations, as indicated by boxes.C: Number of CD11b⁺Ly6C^hi inflammatory monocytes in obstructed kidneys from Lck-Cre Agtra^flox/flox [total knockout (TKO)] and wild-type (WT) mice. D–F: mRNA expression of tumor necrosis factor (TNF)-α, chemokine (C-C motif) ligand 2 (CCL2), and early growth response (EGR)-2 in wild-type macrophages co-cultured with T lymphocytes with and without AT₁ receptor deletion. ^∗P < 0.05. FSC-A, forward scatter area; FSC-H, forward scatter height; KO, knockout; SSC-A, side scatter area.