Supplemental Figure S2.

Sample-wise distribution metrics for total and usable consensus sequences. Each consensus sequence was derived from a group of sequencing reads that share the same unique molecular barcode. Note that the number of sequencing reads in each group indicates the size of each molecular barcode family. Total consensus sequences include all consensus sequences derived from molecular barcode families with a size of ≥1; however, usable consensus sequences include only those derived from families with a size of ≥2. On average, 87% of the total consensus sequences (181,670 of 206,914) were usable for identifying variants. PR, positive reference.