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. 2018 Sep 15;74(6):794–801. doi: 10.1093/gerona/gly209

Figure 2.

Figure 2.

Effects of age and HIV infection on MINCLE-induced cytokine production in CD14+CD16+ Inflammatory monocytes. The cohort consists of HIV-negative young adults (n = 21), HIV-negative older adults (n = 24), HIV-positive young adults (n = 14), HIV-positive older adults (n = 22). (a) Dot plots of percent change in production of interleukin IL-10, IL-12, IL-6, and tumor necrosis factor-α (TNF-α) compared to baseline in the indicated groups, as assessed by intracellular cytokine staining, after stimulation with trehalose-6,6-dibehenate. The following comparisons, indicated by asterisks, were statistically significant using a Wilcoxon two-sample test with t approximation, with an additional false discovery rate adjustment: HIV-negative older adults versus young adults, IL-6 (p = .005), IL-12 (p = .007); HIV-positive versus HIV-negative young adults, IL-6 (p =.012), IL-10 (p = .028), IL-12 (p = .001), and TNF-α (p = .001); HIV-positive versus HIV-negative older adults: IL-12 (p = .011). All other comparisons were not significant as observed in this study. (b) Dot plots showing Boolean gating of specific cytokine combinations in the indicated cohorts. The following statistically significant comparisons are shown (indicated by asterisks): HIV-negative older versus young adults, IL-6/IL-12 ( = 0.005); HIV-positive versus HIV-negative young adults, IL-10/IL-12 (p = .001), IL-6/IL-12 (p < .001), IL-10/IL-6 (p = .028), IL-10/IL-12/IL-6/TNF-α (p = .013); HIV-positive versus HIV-negative older adults, IL-10/IL-12 (p = .021).