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. 2019 Apr 12;54(6):1955–1968. doi: 10.3892/ijo.2019.4782

Figure 4.

Figure 4

miRNAs are closely associated with MTDH. (A) RT-qPCR was used to detect the expression levels of 16 miRNAs in PWR-1E, PC-3, LNCaP and DU145 cells. (B) The 16 miRNAs associated with MTDH were screened by DianaTools, miRTarBase, miRWalk and TargetScan (**P<0.01, compared to normal PWR-1E cells). (C) Four representative miRNAs, miR-145-5p, miR-145-3p, miR-499a-5p and miR-22-3p, were selected to perform the following experiments. The inhibitors of these 4 miRNAs were used to determine their effects on the protein expression of MTDH in PWR-1E cells. β-actin served as an internal control. (D) The mimics of these 4 miRNAs were used to determine their effects on the protein expression of MTDH in PC-3, LNCaP and DU145 cells. β-actin served as an internal control. (E) The effects of the inhibitors or mimics of the 4 miRNAs at the protein levels of MTDH in PWR-1E or 3 prostate cancer (PCa) cells are shown as bar diagrams. (F) The effects of the inhibitors or mimics of the 4 miRNAs on the mRNA levels of MTDH in PWR-1E or 3 PCa cells are shown as bar diagrams. Data are shown as the means ± SD from 3 independent experiments [^^P<0.01, compared to mock/negative control (NC) group]. MTDH, metadherin.