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Verification of whether MTDH is a direct target of miR-145-5p, miR-145-3p, miR-499a-5p and miR-22-3p. (A) Putative miR-145-5p, miR-145-3p, miR-499a-5p and miR-22-3p binding sites in the 3′-UTR of MTDH. (B) Relative luciferase activity in 293T cells following co-transfection with wild-type or mutant (mut) MTDH 3′-UTR reporter, which was constructed together with miR-145-5p, miR-145-3p, miR-499a-5p or miR-22-3p (**P<0.01, compared with control + MTDH-3′-UTR group).
