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. 2019 Apr 10;54(6):2080–2094. doi: 10.3892/ijo.2019.4780

Figure 3.

Figure 3

Effects of both catalase and ALDH on cytotoxicity induced on LoVo cells by purified ZmPAO in the presence of spermine. LoVo WT (solid symbols) and LoVo DX (open symbols) cells were incubated with ZmPAO and 12 µM of exogenous spermine up to 60 min at 37°C, in the absence (squares) or presence of 240 U/ml catalase (triangles) or 0.4 U/ml ALDH and 1.8 mg/ml NAD+ (circles). Each point represents the mean ± SEM of 2 independent experiments, with 2 to 5 plates per experiment. Where not shown, error bars lie within symbols. Data are shown on a logarithmic scale. Data were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. **P<0.01, ***P<0.001 and ****P<0.0001 vs. LoVo WT cells incubated with ZmPAO and spermine; †P<0.05, †††P<0.001 and ††††P<0.0001 vs. LoVo DX cells incubated with ZmPAO and spermine. ZmPAO, maize polyamine oxidase; LoVo WT cells, LoVo wild-type cells; LoVo DX cells, LoVo multidrug-resistant cells.