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. 2019 Apr 10;54(6):2080–2094. doi: 10.3892/ijo.2019.4780

Figure 7.

Figure 7

Cell cycle analysis carried out by flow cytometry on LoVo cells. LoVo cells were treated with 12 or 24 µM spermine in the presence of ZmPAO for 60 min at 37°C. At 24 h after the end of the treatment, and incubation at 37°C, followed by the staining with PI, the cells were analyzed by flow cytometry. (A) Representative histograms of sub-G1 analysis performed on LoVo WT and LoVo DX cells using PI staining are shown. The percentage of the sub-G1 cell population is indicated. The histograms have been obtained from 1 out of 2 experiments carried out in the same experimental conditions, which gave similar results. (B) Each bar represents the mean ± SD of sub-G1 cell population of 2 independent experiments. Data were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. *P<0.05 vs. control LoVo WT cells; ††P<0.01 vs. control LoVo DX cells; ‡P<0.05 vs. LoVo WT cells incubated with ZmPAO and spermine. ZmPAO, maize polyamine oxidase; LoVo WT cells, LoVo wild-type cells; LoVo DX cells, LoVo multidrug-resistant cells.