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. 2019 Apr 16;54(6):2095–2105. doi: 10.3892/ijo.2019.4789

Figure 3.

Figure 3

Cell apoptosis as analyzed via Caspase-Glo, TUNEL and flow cytometry assays. (A) Caspase-Glo analysis was performed to detect the apoptosis of cells. *P<0.05, **P<0.01, ***P<0.001 vs. vector or NC. (B) Cells stained with TUNEL and DAPI were considered as apoptotic, *P<0.05 vs. vector or NC (magnification, ×200). (C) Cell apoptosis as analyzed by flow cytometry. *P<0.05 vs. NC, **P<0.01 vs. vector .TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; HOXA6, homeobox A6; vector, vector-only control; NC, negative control; shHOXA6, HOXA6 short hairpin RNA; PI, propidium iodide; FITC, fluorescein isothiocyanate.