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. 2019 Mar 22;54(6):1943–1954. doi: 10.3892/ijo.2019.4761

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Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Downregulation of ILT4/HLA-G expression inhibits the proliferation, migration and invasion of CRC cells. (A) The effects of ILT4/HLA-G knockdown on proliferation were evaluated by Cell Counting Kit 8 assays. The effects of ILT4/HLA-G knockdown on migration were investigated by (B) wound-healing assay (magnification, ×40) and (C) Transwell migration assay (magnification, ×100). (D) The effects of ILT4/HLA-G knockdown on inva sion were measured by Matrigel invasion assays. Magnification, ×100. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. the corresponding NC. The results are presented as the mean ± standard deviation from three independent experiments. HLA-G, human leukocyte antigen G; ILT4, immunoglobulin like transcript 4; NC, negative control; sh, short hairpin.