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. 2019 Mar 22;54(6):1943–1954. doi: 10.3892/ijo.2019.4761

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Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Addition of HLA-G FP activates AKT and ERK signaling, and promotes the proliferation, migration and invasion of HT29 cells. (A) Protein expres sion of ILT4, AKT, p AKT, ERK, p ERK and GAPDH in HT29 following the addition of HLA-G FP. PBS served as a negative control. (B) The effects of HLA-G FP on proliferation were evaluated by Cell Counting Kit 8 assays. The effects of HLA-G FP on migration were detected by (C) wound healing assay (magnification, ×40) and (D) Matrigel invasion assay (magnification, ×100). (E) The effects of HLA-G FP on invasion were measured by Matrigel invasion assays. Magnification, ×100. The results are presented as the mean ± standard deviation from three independent experiments. ^*P<0.05 and ^***P<0.001 vs. cells with 0 ng/ml HLA-G FP. HLA-G, human leukocyte antigen G; ILT4, immunoglobulin like transcript 4; FP, fusion protein; p , phospho ; ERK, extracellular signal regulated kinase; AKT, protein kinase B.