Figure 4.

INKA2 functions as a cancer cell growth inhibitor. (A) Results of the colony formation assay performed with HBC4 (left panel) and U2OS (right panel) cells overexpressing the mock vector or INKA2. The number of colonies in the mock-transfected cells is considered 100%. Error bars are the means ± SD, n=3. (B and C) Differential expression of the (B) INKA2 mRNA in Log₂ RSEM and (C) DNA methylation level at the INKA2 promoter region in TCGA control and tumour samples. Data were analysed with a two-tailed Student’s t-test; ^*P<0.05, ^**P<0.01 and ^***P<0.001. INKA2, inka box actin regulator 2. BRCA, breast invasive carcinoma; COAD, colon adenocarcinoma; KIRC+KIRP, kidney renal clear cell and papillary cell carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; PRAD, prostate adenocarcinoma; THCA, thyroid carcinoma.