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. Author manuscript; available in PMC: 2019 May 16.
Published in final edited form as: Vaccine. 2018 Jul 25;36(35):5325–5332. doi: 10.1016/j.vaccine.2018.06.054

Figure 3. IgHV Germline Usage.

Figure 3

The NT sequence of the IgHV of each clone was assayed In silico for its unmutated common ancestor germline IgHV gene. Clones using the same IgHV germline gene are grouped together, regardless of lineage relationship. Select germline genes are highlighted specific colors. Remaining germline genes are presented in grayscale. A) IgHV germline usage by patient visit day. B) Clones were assessed for binding specificity by both simple and competition ELISA. Clones described as binding at the X-181 HA RBS competed directly with Ab6639, whose crystal structure is published.[16] Clones described as binding on the HA head but not at the RBS bound the trimeric head-only construct but did not compete directly with Ab6639. Clones described as binding the X-181 HA non-head FLsE did bind the Trimeric X-181 HA FLsE construct but not the X-181 HA head-only construct. Most competed directly with CR6261, whose HA-Stem-binding crystal structure is published.[29] Clones of a similar binding epitope were grouped based on their IgHV germline usage, regardless of lineage relationship. The most prominent germline genes are highlighted specific colors. Remaining germline genes are presented in grayscale

* Includes CL6515 [16]

? Includes CL6649 [15].