Fig 1. PCR-RFLP analyses of mpi gene fragments from 6 Leishmania species in Ecuador.

PCR amplification was performed with leishmanial mpi gene-specific primers, and PCR products were digested with (A) HaeIII and (B) HpaI, and resulting restriction fragment patterns were analyzed by agarose gel electrophoresis. 1. L. (V.) guyanensis, 2. L. (V.) panamensis, 3. L. (V.) braziliensis, 4. L. (V.) naiffi, 5. L. (L.) major-like, 6. L. (L.) mexicana.