Table 2. Primer sequences used in this study.

Target gene	Primer		Primer sequence (5’ to 3’)	Expected amplicon size (bp)
cytochrome b	outer	L.cyt-AS	GCGGAGAGRARGAAAAGGC	978
(cyt b)		L.cyt-AR	CCACTCATAAATATACTATA
	inner	L.cyt-S	GGTGTAGGTTTTAGTYTAGG	866
		L.cyt-R	CTACAATAAACAAATCATAATATRCAATT
cytochrome oxidase	outer	L.COII-1S	AACATAGTTCTCATTGCAGA	954
subunit II—NADH		L.COII-1R	ACAMCGRCCAGGTTCTCTAC
dehydrogenase subunit 1	inner	L.COII-2S	AATGCAACATGCAGTTATWA	736
(COII-ND1)		L.COII-2R	AATGAATGTATAACATCAAC
heat shock protein 70	outer	L.HSP-OS	GGGCACGACGTACTCGTGCG	1,931
(hsp70)		L.HSP-OR	AGTCGACCTCCTCGACCTTG
	inner	L.HSP-IS2	CCGTCGTACGTTGCGTTCAC	1,735
		L.HSP-IR2	TGCTCTGGTACATCTTGGTC
	outer*	L.HSP-Ty1S	GGCGAGCGCGCGATGACGAA	847
		L.HSP-OR	AGTCGACCTCCTCGACCTTG
	inner*	L.HSP-Ty2S	CGTTCGACTTGTCCGGCATC	468
		L.HSP-IR2	TGCTCTGGTACATCTTGGTC
mannose phosphate	outer	L.MPI-OS2	GCCTGGGGCAAGRATGCCGC	1,214
isomerase		L.MPI-OR	CTCAAGTCGTTGGTCGACGC
(mpi)	inner	L.MPI-IS2	CGTCCAGCTTCGTGGCRAAG	1,130
		L.MPI-IR2	GCCGTACGGYACCGCAAAGC
6-phosphogluconate	outer	L.6PGD-OS	GAACGACCTCGGYATTATCG	1,346
dehydrogenase		L.6PGD-OR	GACACCAGCTGTCCGTACGG
(6pgd)	inner	L.6PGD-IS	GCCCTGAACATCGCCGAGAA	1,272
		L.6PGD-IR	CGTGTACATGGCGTTGATGT

*The primer sets were used for the PCR-RFLP analysis to differentiate L. (V.) guyanensis from L. (V.) panamensis.