Table 4. Comparison of Leishmania species identification in Ecuador between cyt b sequence analysis and PCR-RFLP analyses of nuclear DNAs.
|
Target gene (analysis) |
Identification* (numbers) | |||
|---|---|---|---|---|
|
cyt b (cloning and sequencing) |
L.g (73) | L.b (14) | L.n (2) | L.mex (3) |
|
mpi, 6pgd, and hsp70 (PCR-RFLP) |
L.g (72) L.g/L.p (1) |
L.b (6) L.g/L.b (3) L.g# (1) L.p# (3) L.g/L.p# (1) |
L.n (2) | L.mex (3) |
*L.g: L. (V.) guyanensis, L.p: L. (V.) panamensis, L.b: L. (V.) braziliensis, L.n: L. (V.) naiffi, L.mex: L. (L.) mexicana, L.g/L.p: a hybrid of L. (V.) guyanensis and L. (V.) panamensis, L.g/L.b: a hybrid of L. (V.) guyanensis and L. (V.) braziliensis
#mito-nuclear discordance