Table 2. Information on the B-cell epitope prediction algorithms employed.

A total of 80 peptides with high antigenic scores, independently predicted from four different B-cell prediction algorithms, were selected for synthesis. AA: amino acid; AUC: area under the curve.

Algorithm	Details	n
ABCPred	Predicts antigenic regions of fixed even length and assigns a score, ranging from zero to one, for the whole predicted k-mer.	20
lbtope	Scans all possible 15mers with one AA offset on all the submitted sequences. A numeric score as well as the probability of correctnesses of each prediction are outputted.	20
EpiQuest-B	Calculates individual scores per AA residue (AGR) and the final peptides scores are ranked by the relative immunogenicity index, which corresponds to the curve (AUC) in a plot residue position vs. AGR.	20
Bepipred	Assigns a score for each AA residue on the submitted sequences. Predicted epitope regions can be of any length ≥ 1 AA with a score greater than the set cut-off (0.35 for default values). The antigenicity of the 16mers was calculated from the AUC in a plot residue position vs. score.	20
	Total	80