Figure 6.

BubR1 loss reduces the number of cells in mitosis. (A and B) Loss of BubR1 does not affect the proportion of S-phase cells marked by 30-min BrdU pulse labeling, among total proliferating aNPCs (BrdU+/Pax6+), in BubR1 CKO at E14.5. (C and D) The total number of mitotic cells across the ventricular lining is significantly reduced in BubR1 CKO. PH3+ cells counted from three sections from each animal are compared between WT and BubR1 CKO (n = 3 for each genotype). (E and F) Cell cycle analyses using flow cytometry after 30-min BrdU incorporation and propidium iodide staining show a reduction of mitotic cells (G2+M) without affecting other cell cycle phases in BubR1 CKO. BubR1 CKO cortices also include a significant proportion of SubG cells.^*P < 0.05. Scale bar, 100 μm.