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ND96 Solution
| Reagent | Grams in 1L | Final Concentration (1X) |
|---|---|---|
| NaCl | 5.61 g | 96 mM |
| KCl | 0.1491 g | 2 mM |
| CaCl2.2H2O | 0.2646 g | 1.8 mM |
| MgCl2.6H2O | 0.2033 g | 1 mM |
| HEPES | 4.766 g | 20 mM |
| Na Pyruvate | 0.276 g | 2.5 mM |
| HEPES | 4.766 g | 20 mM |
| Penicillin-Streptomycin | 100000 U | 100U/mL |
| H2O | Add water to 1L |
Use 4 M NaOH to adjust pH to 7.5–7.6
