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Oocytes Stripping Solution
| Reagent | Grams in 1L | Final Concentration (1X) |
|---|---|---|
| N-Methyl-D-Glucamine | 39.044 g | 200 mM |
| Aspartate | 26.62 | 200 mM |
| HEPES | 4.766 g | 20 mM |
| KCl | 0.1491 g | 2 mM |
| MgCl2.6H2O | 0.2033 g | 1 mM |
| EGTA | 3.804 g | 10 mM |
| H2O | Add water to 1L |
Use 4 M NaOH to adjust pH to 7.4
