Basal Internal Solution (+EGTA)
| Reagent | Grams in 1L | Final Concentration (1X) |
|---|---|---|
| KOH | 7.854 g | 140 mM |
| HEPES | 4.766 g | 20 mM |
| KCl | 0.1491 g | 2 mM |
| EGTA | 0.3804g | 1 mM |
| MeSO3 | 13.314 g | 140 mM |
| H2O | Add water to 1L |
Use MeSO3 to adjust pH to 7.1–7.2
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