Table 1. Quantitative measurements of HSV1 DNA inside of RCs.
Related to Figure 4. Using the values obtained through DNA FISH and ATAC-seq, we can make estimates of the copy number, concentrations, and relative enrichment of the viral DNA compared to the host. All values are calculated based on measurements of cells 6 hpi.
| Table 1 | Genome Size (bp) | Genome Copy number‡ | Total DNA (bp) | Percent of Total DNA‡ | Concentration (bp/μm3 )§ | ATAC-seq read percentage¶ | Fold enrichment over expected** |
|---|---|---|---|---|---|---|---|
| Host Genome* | 3.2 × 109 | 2 | 6.4 × 109 | 99.8 (±0.2) | 9.4 (±1.6) x106 |
75.8 (±10.4) | 0.8 (±0.1) |
| Viral DNA | 1.5 × 105 | 82 (±105) | 1.3 (±1.6) x107 |
0.2 (±0.2) | 3.9 (±5.8) x104 |
24.2 (±10.4) | 130 (±170) |
| Rel. Diff.† | 2.1 × 104 | 513 (±658) | 240 (±369) |
All values are the Mean (±S.D.).
*. Assuming karyotypically normal human cell; †. relative difference = Human/HSV1; ‡. Under experimental conditions of MOI = 1; §. Concentration assuming nucleus volume taken from Monier et al. (2000); ¶. based on total reads mapped from each organism, n = 3; ** Fold enrichment = ATAC seq read percentage/Percent of Total DNA.