Extended Data Fig. 1 |. Deletion efficacy of Ythdf1^−/− mice.

a-b, Off-target analysis of the CRISPR/Cas9 system in Ythdf1^−/− mice. (a) Ythdf1 sgRNA targeting sites and four putative off-target sites were amplified. (b) PCR products of Ythdf1^−/− mice and WT mice were mixed and digested by T7EI. The PCR product from WT mice was used as negative control. c, Immunoblot assays were shown to validate YTH protein expression level changes in Ythdf1^−/− DCs. Data are representative of one experiment (a, b) and two independent biological replications for (c).