Extended Data Fig. 2 |. Characterizations of immune phenotypes of Ythdf1-deficient mice.

(a) data points for Fig. 1a. (b) WT or Ythdf1^−/− mice were injected s.c. with 10⁶ B16-OVAcells. Tumor survival were monitored. Mice with tumor volumes less than 200 mm³ are considered to be surviving. One of three representative experiments is shown. (c) data points for Fig. 1b. d-h, WT or Ythdf1^−/− mice were injected s.c. with 10⁶ B16-OVAcells. (d,e) The frequency of tumor infiltrating MDSC (Ly6c⁺CD11b⁺) cells was assessed 12 days post tumor inoculation. (f,g) The percentages of Treg in spleen, draining lymph node (DLN) and tumor are shown. (h) Degranulation of tumor NK cells in response to in vitro re-stimulation with PMA/ionomycin. (i) data points for Fig. 1d. Data are representative of two independent experiments (a, c). n, numbers of mice. Data are mean ± s.e.m. and were analyzed by two-tailed unpaired Student’s t-test for (a, c, e, g-i) and two- tailed log-rank (Mantel-Cox) test (b).