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. Author manuscript; available in PMC: 2019 May 16.
Published in final edited form as: Drug Metab Dispos. 2003 Jun;31(6):705–713. doi: 10.1124/dmd.31.6.705

FIG. 1. Preincubation of cisplatin with GSH, Cys-Gly, or NAC; effect of preincubation on the toxicity of cisplatin toward LLC-PK1 cells.

FIG. 1.

Cisplatin was preincubated with equimolar GSH, Cys-Gly, or N-acetylcysteine in HBSS with 5 mM HEPES (pH 7.2) at 37°C for the times indicated to allow for the spontaneous formation of cisplatin conjugates. The toxicity of the mixture was assayed by treating LLC-PK1 cells for 3 h with 100 µM cisplatin preincubated with GSH (A), 100 µM cisplatin preincubated with Cys-Gly (B), or 100 µM cisplatin preincubated with NAC (C). The number of viable cells was determined at 72 h. Bars, values are the mean of three measurements ± S.D.