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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Cancer Res. 2019 Mar 25;79(10):2649–2661. doi: 10.1158/0008-5472.CAN-18-2331

Figure 2. Identification of MITF as a transcriptional target of p300 via global gene expression profile analysis.

Figure 2.

(A) Genes differentially expressed following EP300 gene silencing in human melanoma cells. Two hundred and fifty overlapping genes in both p300-depleted WM983B and SK-Mel5 cells were identified as p300 downstream targets. (B) Canonical pathways regulated by p300 in melanoma cells. Ingenuity Pathway Analysis (IPA) of the p300 downstream target genes revealed that pathways associated with cell proliferation are altered in p300 depleted melanoma cells. (C) Identification of potential upstream regulators of p300 target genes by IPA. MITF was found as one of the upstream regulators of p300 target gene expression. (D) Number of known MITF-target genes that are also included in the p300-downstream effector genes. IPA identified 33 genes from the p300 target gene set as known MITF target genes. Decreased transcript levels of MITF and its target genes were further verified in p300-depleted melanoma cells via qRT-PCR. (E) Western blot analysis evaluated PAX3 and SOX10 levels in p300-silenced melanoma WM983B and SK-Mel 5 cells.