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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Cancer Res. 2019 Mar 25;79(10):2649–2661. doi: 10.1158/0008-5472.CAN-18-2331

Figure 3. MITF rescues the p300-dependent growth phenotype in p300 knockdown melanoma cells.

Figure 3.

(A) Illustrative western blot analysis validating stable knockdown of MITF by shRNAs in melanoma cells. WM983B cell line is shown. (B) Morphological changes present in melanoma cell lines after MITF depletion. (C to F) Cell proliferation assay demonstrating decreased cell growth rates in the melanoma cell lines following stable depletion of MITF. (G) Representative western blot analysis validating ectopic re-expression of MITF in p300 stable knockdown cells. p300 knockdown demonstrates inhibition of MITF expression (also shown in the Figure 2E). Plasmid vectors expressing MITF (pCW45-MITF) and control GFP (pCW45-GFP) were transiently transfected in p300-depleted melanoma cells (WM983B). (H) Relative cell growth assay of p300-depleted WM983B and 1205Lu melanoma cells following transient re-expression of MITF versus GFP control. Error bars, SD; ***p<0.001.