Fig. 1.

Schematic of immunophenotyping of RCD. Live (including early apoptotic) and dead (including late apoptotic) cells were gated from a Zombie NIR vs. Caspase-3-BV650 dot-plot (a). Live and dead necroptotic cells were defined as Caspase-3^− ve/RIP3^{high + ve}, early or late apoptotic (Caspase-3^+ ve/RIP3^− ve), RIP1-dependent apoptotic (RIP1-APO, Caspase-3^+ ve/RIP3^+ ve) and Double Negative (DN, Caspase-3^− ve/RIP3^− ve) (b, c). Then each of these live and dead cell populations were then gated on a H2AX vs. PARP dot-plots to show the incidence of DDR (H2AX^+ ve/PARP^− ve), hyper-activation of PARP (H2AX^+ ve/PARP^+ ve), parthanatos (H2AX^− ve/PARP^+ ve) and QN (H2AX^− ve/PARP^− ve), (d, e)