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. 2019 Feb 20;24(5):453–464. doi: 10.1007/s10495-019-01528-w

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Autophagy assay. Untreated Jurkat cells or after CQ ± zVAD treatment for 24 h were analysed by gating on live and dead cells from a Zombie NIR vs. Caspase-3-BV650 dot-plot with live and dead of untreated cells (a, b), CQ treated (c, d) or CQ + zVAD treated Jurkat cells (e, f) were then analysed for autophagy (PERK^− ve/LC3B^+ ve) and ER stress (PERK^+ ve/LC3B^− ve) from a LC3B v PERK do-plot. n = 3, student t test NS (not significant), P < 0.05*, P < 0.01**, P < 0.001***, with arrows indicating change compared to untreated cells